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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
21/02/2014 |
Actualizado : |
29/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
GAIERO, P.; MAZZELLA, C.; VILARÓ, F.; SPERANZA, P.; DE JONG, H. |
Afiliación : |
PAOLA GAIERO, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; Laboratory of Genetics, Wageningen University, Netherlands; CRISTINA MAZZELLA, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO SPERANZA, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; HANS DE JONG, Laboratory of Genetics, Wageningen University, Netherlands. |
Título : |
Pairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Euphytica, 1 July 2017, Volume 213, Issue 7, Article number 137. OPEN ACCESS. |
ISSN : |
0014-2336 |
DOI : |
10.1007/s10681-017-1922-4 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 29 December 2016 / Accepted: 29 May 2017 / Published online: 14 June 2017. |
Contenido : |
ABSTRACT.
Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental species. As a consequence, we were not able to trace the course of the S. commersonii chromosomes in the successive introgressive hybridisation backcross generations. Our results strongly point at a high genomic similarity between the homoeologous chromosomes promising high suitability of S. commersonii in introgressive hybridisation breeding of potato.
© 2017, The Author(s). MenosABSTRACT.
Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental spe... Presentar Todo |
Palabras claves : |
BAC FISH; GISH; Introgressive hybridisation; Pairing behaviour; Potato wild relatives. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
URL : |
https://link.springer.com/content/pdf/10.1007%2Fs10681-017-1922-4.pdf
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Marc : |
LEADER 02814naa a2200265 a 4500 001 1012827 005 2019-10-29 008 2017 bl uuuu u00u1 u #d 022 $a0014-2336 024 7 $a10.1007/s10681-017-1922-4$2DOI 100 1 $aGAIERO, P. 245 $aPairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids.$h[electronic resource] 260 $c2017 500 $aArticle history: Received: 29 December 2016 / Accepted: 29 May 2017 / Published online: 14 June 2017. 520 $aABSTRACT. Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental species. As a consequence, we were not able to trace the course of the S. commersonii chromosomes in the successive introgressive hybridisation backcross generations. Our results strongly point at a high genomic similarity between the homoeologous chromosomes promising high suitability of S. commersonii in introgressive hybridisation breeding of potato. © 2017, The Author(s). 653 $aBAC FISH 653 $aGISH 653 $aIntrogressive hybridisation 653 $aPairing behaviour 653 $aPotato wild relatives 700 1 $aMAZZELLA, C. 700 1 $aVILARÓ, F. 700 1 $aSPERANZA, P. 700 1 $aDE JONG, H. 773 $tEuphytica, 1 July 2017, Volume 213, Issue 7, Article number 137. OPEN ACCESS.
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INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
16/10/2017 |
Actualizado : |
13/08/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
CRUZ, G.; BAETHGEN, W.; BARTABURU, D.; BIDEGAIN, M.; GIMÉNEZ, A.; METHOL, M.; MORALES, H.; PICASSO, V.; PODESTA, G.; TADDEI, R.; TERRA, R.; TISCORNIA, G.; VINOCUR, M. |
Afiliación : |
G. CRUZ, Universidad de la República (UdelaR)/ Facultad de Agronomía; WALTER E. BAETHGEN, Universidad de Columbia/ IRI (International Research Institute for Climate and Society); D. BARTABURU, IPA (Instituto Plan Agropecuario); M. BIDEGAIN, INUMET (Instituto Uruguayo de Meteorología); AGUSTIN EDUARDO GIMÉNEZ FUREST, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; M. METHOL, MGAP/ OPYPA (Oficina de Programación y Política Agropecuaria).; H. MORALES, IPA (Instituto Plan Agropecuario); V. PICASSO, Universidad de la República (UdelaR)/ Facultad de Agronomía; Universidad de Wisconsin-Madison; G. PODESTA, University of Miami, Rosenstiel School of Marine and Atmospheric Science; R. TADDEI, Federal University of São Paulo (UNIFESP); R. TERRA, Universidad de la República (UdelaR)/ Facultad de Ingeniería; GUADALUPE TISCORNIA TOSAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; M. VINOCUR, Universidad Nacional de Río Cuarto (UNRC), Facultad de Agronomía y Veterinaria. |
Título : |
Thirty years of multi-level processes for adaptation of livestock production to droughts in Uruguay. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Weather, Climate, and Society (WCAS), 2017. |
DOI : |
10.1175/WCAS-D-16-0133.1 |
Idioma : |
Inglés |
Notas : |
Publicado en el 2018: WCAS, jan.,2018, v. 10, p. 59-74. Article history: Received: 22 December 2016; Final Form: 2 Octobre 2017; Published online: 13 December 2017. |
Contenido : |
ABSTRACT.
Most countries lack effective policies to manage climate risks, despite growing concerns with climate change. We analyzed the policy evolution from a disaster management to a risk management approach, using as a case study four agricultural droughts that impacted Uruguay?s livestock sector in the last three decades. A transdisciplinary team of researchers, extension workers, and policy makers agreed on a common conceptual framework for the interpretation of past droughts and policies. The evidence presented shows that the set of actions implemented at different levels when facing droughts were mainly reactive in the past but later evolved to a more integral risk management approach. A greater interinstitutional integration and a decreasing gap between science and policy were identified during the period of study. Social and political learning enabled a vision of proactive management and promoted effective adaptive measures. While the Government of Uruguay explicitly incorporated the issue of adaptation to climate change to its agenda, research institutions also fostered the creation of interdisciplinary study groups on this topic, resulting in new stages of learning. The recent changes in public policies, institutional governance and academic research, have contributed to enhance the adaptive capacity of the agricultural sector to climate variability, and in particular to drought. This study confirms the relevance and need to work within a transdisciplinary framework to effectively address the different social learning dimensions, particularly those concerning the adaptation to global change.
@2017 American Meteorological Society MenosABSTRACT.
Most countries lack effective policies to manage climate risks, despite growing concerns with climate change. We analyzed the policy evolution from a disaster management to a risk management approach, using as a case study four agricultural droughts that impacted Uruguay?s livestock sector in the last three decades. A transdisciplinary team of researchers, extension workers, and policy makers agreed on a common conceptual framework for the interpretation of past droughts and policies. The evidence presented shows that the set of actions implemented at different levels when facing droughts were mainly reactive in the past but later evolved to a more integral risk management approach. A greater interinstitutional integration and a decreasing gap between science and policy were identified during the period of study. Social and political learning enabled a vision of proactive management and promoted effective adaptive measures. While the Government of Uruguay explicitly incorporated the issue of adaptation to climate change to its agenda, research institutions also fostered the creation of interdisciplinary study groups on this topic, resulting in new stages of learning. The recent changes in public policies, institutional governance and academic research, have contributed to enhance the adaptive capacity of the agricultural sector to climate variability, and in particular to drought. This study confirms the relevance and need to work within a transdisciplinary framew... Presentar Todo |
Palabras claves : |
AGRICULTURA; CLIMATE CHANGE; CLIMATE VARIABILITY; DROUGHT; POLICY; PUBLIC POLICIES; SOCIAL SCIENCE; SOUTH AMERICA. |
Thesagro : |
CAMBIO CLIMÁTICO; URUGUAY. |
Asunto categoría : |
P40 Meteorología y climatología |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10993/1/wcas-d-16-0133.1.pdf
https://journals.ametsoc.org/doi/pdf/10.1175/WCAS-D-16-0133.1
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Marc : |
LEADER 02913naa a2200409 a 4500 001 1057659 005 2018-08-13 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1175/WCAS-D-16-0133.1$2DOI 100 1 $aCRUZ, G. 245 $aThirty years of multi-level processes for adaptation of livestock production to droughts in Uruguay.$h[electronic resource] 260 $c2017 500 $aPublicado en el 2018: WCAS, jan.,2018, v. 10, p. 59-74. Article history: Received: 22 December 2016; Final Form: 2 Octobre 2017; Published online: 13 December 2017. 520 $aABSTRACT. Most countries lack effective policies to manage climate risks, despite growing concerns with climate change. We analyzed the policy evolution from a disaster management to a risk management approach, using as a case study four agricultural droughts that impacted Uruguay?s livestock sector in the last three decades. A transdisciplinary team of researchers, extension workers, and policy makers agreed on a common conceptual framework for the interpretation of past droughts and policies. The evidence presented shows that the set of actions implemented at different levels when facing droughts were mainly reactive in the past but later evolved to a more integral risk management approach. A greater interinstitutional integration and a decreasing gap between science and policy were identified during the period of study. Social and political learning enabled a vision of proactive management and promoted effective adaptive measures. While the Government of Uruguay explicitly incorporated the issue of adaptation to climate change to its agenda, research institutions also fostered the creation of interdisciplinary study groups on this topic, resulting in new stages of learning. The recent changes in public policies, institutional governance and academic research, have contributed to enhance the adaptive capacity of the agricultural sector to climate variability, and in particular to drought. This study confirms the relevance and need to work within a transdisciplinary framework to effectively address the different social learning dimensions, particularly those concerning the adaptation to global change. @2017 American Meteorological Society 650 $aCAMBIO CLIMÁTICO 650 $aURUGUAY 653 $aAGRICULTURA 653 $aCLIMATE CHANGE 653 $aCLIMATE VARIABILITY 653 $aDROUGHT 653 $aPOLICY 653 $aPUBLIC POLICIES 653 $aSOCIAL SCIENCE 653 $aSOUTH AMERICA 700 1 $aBAETHGEN, W. 700 1 $aBARTABURU, D. 700 1 $aBIDEGAIN, M. 700 1 $aGIMÉNEZ, A. 700 1 $aMETHOL, M. 700 1 $aMORALES, H. 700 1 $aPICASSO, V. 700 1 $aPODESTA, G. 700 1 $aTADDEI, R. 700 1 $aTERRA, R. 700 1 $aTISCORNIA, G. 700 1 $aVINOCUR, M. 773 $tWeather, Climate, and Society (WCAS), 2017.
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